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. 2008 Aug 15;47(36):9514–9521. doi: 10.1021/bi800747e

Figure 2.

Figure 2

SDS−polyacrylamide gel electrophoresis of kinesin preparations after purification to remove proteins lacking the six-histidine tag: coexpressed K401 and K401-BIO-H6 (R210K) (lane 1, lower and upper bands, respectively); K401-BIO-H6 (R210K) alone (lane 2); K401-BIO-H6 alone (lane 3); and K401 alone (lane 4). The lane 4 sample was loaded in 30-fold volumetric excess relative to lane 1 to confirm that removal of the untagged homodimer is essentially complete. Diagrams show the protein species expected in each sample using the same symbols as in Figure 1. Scale (left): molecular weight markers.