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. Author manuscript; available in PMC: 2009 Sep 5.
Published in final edited form as: Cell. 2008 Sep 5;134(5):743–756. doi: 10.1016/j.cell.2008.07.021

Figure 6. Rare XBP1 variants are hypomorphic.

Figure 6

A. MODE-K cells were transfected with UPRE-luciferase and unspliced hXBP1u expression plasmids encoding the rare, IBD-associated minor alleles XBP1snp8 (M139I) and XBP1snp17 (A162P) and treated with 1 µg/ml Tm. Values represent luciferase activities normalized to cotransfected Renilla reporter. B. Experiments as in (a), with indicated amounts of spliced hXBP1s cDNA variants C. Transduction efficiency measured by FACS of XBP1−/− MEF cells reconstituted with human XBP1 wildtype or SNP variants bi-cistronic retroviral vectors (RVGFP) (MFI, mean fluorescence intensity). D. XBP1s protein levels were determined by western blot of Tm-treated cells (*, non-specific band). E. ERdj4 and EDEM mRNA levels (normalized to β-actin mRNA expression) in untreated (NT) or Tm (1 µg/ml)-treated cells for 6 hrs.