Figure 3.

Rerouting of metabolic fluxes. (Black) Flux distribution for the complete gene set. (Red) zwf^- mutant. Biomass yield is 99% of the results for the full metabolic genotype. (Blue) zwf^- pnt^- mutant. Biomass yield is 92% of the results for the full metabolic genotype (see text). The solid lines represent enzymes that are being used, with the corresponding flux value noted. The fluxes [substrates converted/h per g-dry weight (DW)] were calculated by using FBA with the input parameters of glucose uptake rate (β_glucose = 6.6 mmol glucose/h per g-DW) and oxygen uptake rate (β_oxygen = 12.4 mmol oxygen/h per g-DW) (41).