FIGURE 7.

Three-dimensional structure of human PEDF (from Protein Data Bank ID 1IMV) to illustrate the location of the HA-binding site. The two structures are rotated about 180° from each other with highlighted positions of single alterations made in this study. In blue are basic amino acids Lys¹⁴⁶, Lys¹⁴⁷, and Arg¹⁴⁹ residues located in a turn between β-strand s2A and α-helix E, and Lys¹⁸⁹, Lys¹⁹¹, Lys¹⁹⁴, and Arg¹⁹⁷ located in another turn between α-helix F and β-strand s3A, both within BX₇B HA-binding sites; in red are acidic amino acids Asp²⁵⁶, Asp²⁵⁸, and Asp³⁰⁰ corresponding to the collagen-binding site. P2 corresponds to the residue next to the homologous serpin reactive site, P1; and N– in yellow indicates the position of the amino-end terminus of the polypeptide in the three-dimensional structure corresponding to position 26. Structures were visualized and reproduced using Cn3D (NCBI).