TABLE 2.
Thermodynamic parameters derived from isothermal titration calorimetry experiments
Averages of two experimental titrations for each protein are given with standard deviations. ΔG calculated using ΔG° = −RT In (KD −1). TΔS° calculated using ΔG° = ΔH° - TΔS°, where T = 303 K.
| Protein/U20 | KD | ΔG° | ΔH° | −TΔS° |
|---|---|---|---|---|
| nm | kcal mol−1 | kcal mol−1 | kcal mol−1 | |
| wtU2AF65R123 | 820 ± 9 | −8.45 ± 0.02 | −69.5 ± 0.3 | 61.0 ± 0.3 |
| ptbU2AF65R123 | 975 ± 45 | −8.34 ± 0.02 | −52.8 ± 5.0 | 44.4 ± 5.0 |
| dU2AF65R123 | 697 ± 128 | −8.55 ± 0.11 | −67.6 ± 6.1 | 59.0 ± 6.1 |