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. 2008 Nov 28;283(48):33674–33684. doi: 10.1074/jbc.M804288200

FIGURE 2.

FIGURE 2.

Enhanced detection and resolution of disaccharides. A, the accumulated XIC for disulfated disaccharide ions present in equimolar mixtures of untagged D2H6, D0S6, D2S0, and D2S6 are shown. The accumulative XIC is the summation of individual ionic currents for the free molecular ion, [M-H]-1 (m/z = 496), the adduction ion formed with the ion-pairing reagent, [M-2H+DBA]-1 (m/z = 625), and in-source desulfation of D2S6 [M-H-SO3]-1 (m/z = 496). The two isobaric isomers D0S6 and D2S0 did not resolve. B, the accumulated XIC for D2H6, D0S6, D2S0, and D2S6 tagged with [13C6]aniline is shown. The accumulative XIC is the summation of individual ionic currents for the free molecular ion, [M-H]-1 (m/z = 573), the adduction ion formed with the ion-pairing reagent, [M-2H+DBA]-1 (m/z = 702), and the in-source desulfation of D2S6 [M-H-SO3]-1 (m/z = 573). D0S6 and D2S0, which were not resolved in their underivatized state, are resolved as aniline derivatives. C, corresponding mass spectrum for underivatized D0S6 and D2S0 showing the free molecular ions, [M-H]-1, adduction ions formed with the ion-pairing reagent, [M-2H+DBA]-1, and desulfated in-source fragment ions [M-H-SO3]-1. D, the corresponding mass spectra for D0S6 and D2S0 tagged with [13C6]aniline.