FIGURE 2.

Inhibitory effects of the helical peptide inhibitors on γ-secretase. A, inhibitory profiles of helical peptides on γ-secretase cleavage products of APP-based recombinant substrate C100FLAG. Solubilized membrane fractions from γ-30 cells were incubated for 2 h in the presence of 1 μm of the helical peptide inhibitors. Aβ was detected with anti-Aβ antibody 6E10, and AICD-FLAG was detected with anti-FLAG antibody M2. Ctrl, control. B, inhibitory profiles of Cpds 2, 5, 10, and 15. Solubilized membrane fractions from γ-30 cells were incubated for 2 h in the presence of various concentrations of Cpds 2, 5, 10, and 15. The results shown are the average values of two independent experiments.