FIGURE 3.

Coactivators binding is interdependent at ADH2 and FBP1. ChIP for coactivators in gcn5Δ (RBY110, RBY115, RBY116, and RBY155) (A); ada1Δ (RBY106, RBY108, RBY111, RBY113, and RBY154) (B); snf2Δ (RBY109, RBY112, RBY117) (C); and med17 ts (RBY119, RBY120, RBY121) at ADH2 (light bars) and FBP1 (black bars) (D). Data for coactivator occupancy in a WT strain was obtained using RBY3, RBY5, RBY8, RBY9, and RBY11. Values are expressed as the -fold over the WT repressed (R) value, as in Fig. 1A (see “Experimental Procedures” for details). WT data is shown for 4-h derepression (no error bar) or an average of 4- and 6-h derepression (with error bars representing the standard deviation) and data for all mutant strains is based on the average for 4- and 6-h derepression. Error bars of the mutant values indicate the standard deviation of at least three independent biological replicates.