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FRET images for ECFP-HSL EYFP-AFABP/aP2 complex formation. Fluorescence resonance energy transfer (colored magenta as described in the legend to Fig. 2) between ECFP-HSL and the designated AFABP/aP2 mutants in forskolin-stimulated C8PA cells is shown. Each AFABP/aP2 mutant is expressed as a fusion protein with EYFP but is referred to in the figure simply by the AFABP/aP2 amino acid substitution. The scale bar represents 5 μm.
