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. 2008 Nov 28;283(48):33650–33657. doi: 10.1074/jbc.M805927200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Surfactant proteins expression. A, mRNA levels for surfactant-associated proteins (SP-A, SP-B, SP-C, and SP-D). Each expression level is expressed relative to HGPRT. B, immunoblot analysis of surfactant-associated proteins (SP-A, SP-B, SP-C, and SP-D) in lung homogenates from control and HIF1α^Δ/Δ pups (n = 3). β-Actin (actin) was used as loading control. C, immunohistochemical staining of SP-B (left panels) and SP-C (right panels) in lung sections from control (upper panels) and HIF1α^Δ/Δ (lower panels) pups. HIF1α^Δ/Δ pups show decreased expression of SP-B and SP-C as compared with control littermates. The asterisk indicates a significant difference (p value < 0.05) based on a Student's two-tailed t test for samples of unequal variance. All doxycycline treatments were for at least 12 days.