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Interaction of microprotein S with PC/PS-bound DEGR-fXai. Samples containing DEGR-fXai (initially 200 nm) in buffer A were titrated with PC/PS vesicles (not shown) up to a concentration of 50 μm before the addition of microprotein S (open squares) or thrombin-cleaved protein S (open triangles). rO is the anisotropy of PC/PS-bound DEGR-fXai, whereas r is the anisotropy of the dansyl moiety in DEGR-fXai at any point in the subsequent protein titration.
