FIGURE 9.
Ectopic Expression of miR-1 in A549 cells inhibits tumor growth in athymic nude mice. miR-1-expressing A549 cells (clone 2) and vector-transfected cells were trypsinized and counted and then mixed with 50% Matrigel, and 2 × 106 cells were injected subcutaneously to the left and right flanks, respectively, of nude mice. After 4 weeks, tumors were excised and analyzed. A, photograph of tumors developed in six mice. B, average volume of tumors developed in six nude mice ± S.D. C, average weight of tumors developed in six nude mice ± S.D. D, real time RT-PCR analysis of miR-1 in tumors isolated from mouse 3. The results are means of a triplicate assay ± S.D. E, Western blot analysis of miR-1 targets in tumor tissue extracts from mouse 3. Whole cell extracts were subjected to immunoblot analysis with specific antibodies. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.