Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Exp Hematol. 2008 Aug;36(8):947–956. doi: 10.1016/j.exphem.2008.06.005

Fig. 3. Effects of different pre-stimulation times vs. LV exposure period for freshly isolated CB CD34+ cells.

Fig. 3

Freshly isolated CB CD34+ cells were pre-stimulated for 0, 1, 2, 4, 8 and 16 hours individually with cell growth factors (SFT), and cells were analyzed at 72 hours after transduction. Absolute numbers of GFP+CD34+CD38− cells after exposure to virus for 5 (A) or 16 hours (B) were determined by scoring CD38− cells in the gated GFP+CD34+ cell population. Cell viability, after cells were exposed to virus for 5 (C) or 16 (D) hours, was determined by FACS. Transduction efficiency of CD34+ cells exposed for 5 (E) or 16 hours (F) was assessed by determining the percentage of GFP+ as well as CD34+ cells by FACS analysis.