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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Cell Sci. 2008 Sep 30;121(Pt 20):3445–3458. doi: 10.1242/jcs.031484

Figure 6. Imaging of EGF and EEA1.

Figure 6

A. COS-7 cells expressing GFP-EEA1 (green) were exposed to 50 ng/ml Alexa568-EGF (red) and imaged for the times after ligand addition indicated in each panel. After 3 min cells were washed and imaged by TIRF-M with an incident angle set to visualize 300 nm from the coverslip. Co-localized voxels are depicted in white, and indicated with arrowheads. Arrows point to the appearance of a ring-like structure containing EEA1, to which EGF-containing vesicles appear to attach. B. Higher resolution series depicting the association of EGF-containing vesicles (arrow) with EEA1-enriched endosomes. C. Optical sections at 250 nm intervals through the entire cell volume were projected into a single 2D images.