Figure 3.

Infectious BPV-1 can be produced in organotypic rafts grafted on nude mice. Examples of an uninfected (a and b) and a BPV-1 infected (d and e) xenograft are shown. The dermal equivalent contained BALB/BPV fibroblasts that resulted in a fibroma in both cases. a and d show the gross pathology of the graft, and b and e show an H&E-stained section. The flanking mouse skin can be observed at the margins. The plates shown in c and f are 100-mm dishes of C127 cells stained with methylene blue. Foci derived from infectious BPV-1 obtained from an infected xenograft can be observed on the plate in f.