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In situ hybridization for amplified BPV-1 DNA. Examples of grafts with (e and f) and without (a–c) fibroma (because of BALB/BPV fibroblasts) and either uninfected (a), transfected with viral DNA (b and e), or infected with a wart extract (c and f). d shows a natural bovine fibropapilloma. Positive cells containing viral DNA are stained with 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium and counterstained with eosin.
