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. Author manuscript; available in PMC: 2009 Oct 3.
Published in final edited form as: Cell. 2008 Oct 3;135(1):61–73. doi: 10.1016/j.cell.2008.07.043

Figure 6.

Figure 6

The role of SOCS3 for the anti-obesity phenotype of AGRP-IKKβlox/lox mice. A. Relative mRNA levels of SOCS3 in the hypothalamus of HFD- and chow-fed Nestin-IKKβlox/lox mice and IKKβlox/lox mice. B. Relative mRNA levels of SOCS3 in the hypothalamus of chow-fed IKKβlox/lox mice and AGRP-IKKβlox/lox mice that received third-ventricle injections of leptin. (n = 4–6 per group; *p<0.05, **p<10−2, ***p<10−3). C. SOCS3 immunostaining in the MBH of chow- vs. HFD-fed C57BL/6 mice. D. Immunostaining of Cre (green) and SOCS3 (red) in chow-fed AGRP-IKKβlox/lox mice following intra-MBH injections of SOCS3 lentivirus. E & F. SOCS3 lentivirus (SOCS3) or GFP lentivirus (GFP) was injected into the MBH of HFD-fed AGRP-IKKβlox/lox mice; body weight (E) and HFD intake (F) of injected mice were recorded. (n = 4–8 per group; *p<0.05, **p<0.01). Bar = 50 µm. A–F. IKKβl/l: IKKβlox/lox mice; N/IKKβl/l: Nestin-IKKβlox/lox mice; A/IKKβl/l: AGRP-IKKβlox/lox mice.