Figure 1. Purity of CLPs and pre-proB cells after sorting.

CLPs (A) and pre-proB cells (B) were doubly sorted as Lin⁻IL-7Rα⁺Thy-1⁻Sca-1^loc-Kit^lo and B220⁺CD43⁺CD19⁻NK1.1⁻Ly-6C⁻, respectively as shown previously (9, 17). After sorting, we examined the purity of the population on FACS. Shown FACS plots are pre-gated on the PI⁻ fraction to exclude dead cells. Since both Lin⁺ and PI⁺ cells were gated out in the same PE/Cy5 channel in CLP sorting, Lin expression is not shown in (A). After sorting CLPs and pre-proB cells twice, the purity of the populations is over 99%.