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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: J Immunol. 2008 Jul 1;181(1):383–392. doi: 10.4049/jimmunol.181.1.383

Figure 8. Dispensability of IL-7 in the transition from the pre-proB to proB stage.

Figure 8

(A) Requirement of IL-7 in the stage transition from the pre-proB to proB stage. Pre-proB cells were purified from WT mice and cultured on PA6 in the presence or absence of IL-7. Although CD19 expression was observed after the culture in the absence of IL-7, CD19 expression levels were constantly lower than the cells cultured with IL-7. The mean ± S.D. from more than 6 samples from two independent analyses were indicated in the FACS plots. (B) EBF expression level was examined in proB cells derived from pre-proB cells after the culture shown in Figure 6C. B220+CD19+ proB cells were sorted from the culture of WT pre-proB cells in the presence (grey) and absence (black) of IL-7. EBF expression was examined by quantitative RT-PCR. The fold expression of EBF in proB cells was calculated against the EBF expression in WT pre-proB cells (day 0). (C) ProB cell numbers after the culture in the presence (grey) and absence (black) of IL-7. The bars are shown as means of triplicate wells ± S.D. *, p < 0.001, calculated by student’s t test.