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. 2008 Nov 25;6(11):e292. doi: 10.1371/journal.pbio.0060292

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–F) Drosophila cells with or without oleic acid stained with BODIPY493/503 to detect lipid. Control cells not treated with dsRNA (A and D) or cells incubated with dsRNAs targeting Arf79F (an Arf1 homolog) (B and E) or αCop (C and F) are shown.

(G) All COPI, and several COPII members as well as additional Arfs, were retested using independent dsRNAs and gave similar results. Results and number of dsRNAs present in the primary screen (including oleic acid) and retests are given.

(H) Dose response of Drosophila S3 cells to Exo1 (structure inset) showing the %-activity derived either from the lipid specific signal (filled circles [•]) or the lipid/cell ratio (open circles [○]). Percent activity refers hereby to the changes of lipid storage relative to Triacsin C treatment, which decreases lipid storage by blocking TG synthesis (this is analogous to the mdy controls in RNAi experiments, see Material and Methods). Increased activity indicates increased lipid storage, which increased with concentration.

Scale bar in (A) represents 10 μm.