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. 2008 Nov 18;6(11):e280. doi: 10.1371/journal.pbio.0060280

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© 2008 Dethlefsen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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OTU abundance is shown on a logarithmic scale on the vertical axis; OTUs (defined as described in Methods) are listed in rank order along the horizontal axis. The dashed horizontal line at abundance 5 corresponds to an estimated probability of detection of 99%, based on the appearance of a rare OTU in a tag or sequence library according to the Poisson distribution. A comparison of V6, V3, and full-length libraries is shown in (A); the abundance per subject for V6 and V3 refOTUs are shown in (B and C). The maximum V3 refOTU abundance and number of V3 refOTUs are higher for individual A than other participants (C) because three additional samples were analyzed for this subject.