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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Peptides. 2008 Sep 30;29(12):2159–2168. doi: 10.1016/j.peptides.2008.09.014

Fig. 6.

Fig. 6

Nesfatin-1 immunoreactive neurons could be found in the whole ARC. The nesfatin-1 immunopositive neurons were not localized in one particular part of the nucleus, for example the lateral part (A). The double staining for nesfatin-1 (red staining) and Fos (green staining) revealed that numerous Fos-positive neurons in the ARC were also immunopositive for nesfatin-1 (asterisk) after simultaneous peripheral administration of ghrelin and desacyl ghrelin (A and B). Notably, nesfatin-1 immunoreactivity could only be detected in the cytoplasm, whereas the transcription factor Fos was - as expected - localized within the nucleus (C). 3V=third ventricle.