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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Cytometry A. 2008 Sep;73(9):825–833. doi: 10.1002/cyto.a.20609

Figure 6.

Figure 6

Regulation of bud morphology is dependent upon NAP1 expression level. A: Live cells from the indicated strains were examined by DIC microscopy. B: Diagram illustrating the method for calculating relative bud length. C: Images of cells with indicated relative bud length as determined by MIFC. From left to right the overlaid images, SYTOX and brightfield channels are displayed. D: MIFC analysis of bud morphology. A bivariate plot of the aspect ratio and relative bud length from the brightfield channel was generated from the in-focus population. From the gathered images, 20 cells were visually identified as bearing elongated buds and the tagged populations were overlaid on the original plot, as indicated by the red crosses. Boxed region defines cells with elongated buds, where bud length > 1.5 times the bud width and the aspect ratio < 0.5. E: Bar graph indicates the percentage of the population with elongated buds as determined in C, from each of the three indicated strains.