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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2008 Jun 8;111(3-5):171–177. doi: 10.1016/j.jsbmb.2008.05.006

Figure 2. DHEA sulfation by intact T-47D cells.

Figure 2

The T-47D cells were washed twice with PBS then placed in serum-free RPMI1640 without phenol-red in 6 well plates. [3H]-DHEA was added to the medium to a final concentration of 3 µM. The formation of [3H]-DHEA-sulfate was determined in aliquots of the medium by scintillation counting after chloroform extraction to remove unsulfated [3H]-DHEA. DHEA sulfation is expressed as pmol/mg protein. The standard derivation of the [3H]-DHEA-sulfate determinations are included within the points.