Table 2.
DHEA sulfation in breast cancer tissues. Sulfation activity was evaluated as described in Methods in lysate of T47D breast cancer cells and in cytosol and intact nuclei isolated from fresh breast adenocarcinoma specimen. Samples were incubated with 3 µM [3H]-DHEA at 37°C for 20 min to detect SULT2B1b activity. DHEA-sulfate formation was quantified by scintillation counting of an aliquot of the aqueous phase after removal of unsulfated steroids by chloroform extraction. DHEA sulfation is expressed as pmol/mg/min.
| Tissues | DHEA Sulfation Activity (pmol/mg/min) |
|---|---|
| T-47D Cell Lysate | ND |
| Breast Cancer Cytosol | ND |
| Breast Cancer Intact Nuclei | 3.0 |