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Primer locations for PCR amplification and sequencing of ribosomal DNA (rDNA). To obtain DNA fragment including the 5.8 S rDNA and the regions in between the rDNA genes (18S, 5.8S, and 28 S), i.e., the internal transcribed spacer (ITS) 1 and 2, the primers VRF1 and VRF2 were used for PCR amplification.
