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Agarose gel of PCR products obtained using, Aphelenchoides fragariae species-specific primers AfragFl and AfragRl. Templates of total DNA were extracted from Af: A. fragariae, Mi: Meloidogyne incognita; Hs: Heterodera schachtii, Pp: Pratylenchus penetrans, Dd: Ditylenchus dipsaci; and Ce: Caenorhabditis elegans. Lane M contains DNA molecular marker, and lane “-” contains the PCR reaction without DNA template.
