Fig. 4.

Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit; Lane 2: infected A. nidus extracted with Qiagen Dneasy Plant Mini Kit; Lane 3: healthy A. nidus extracted by NaOH lysis; Lane 4: infected A. nidus extracted by NaOH lysis; Lane 5: known A. fragariae DNA template; Lane 6: PCR reaction using NaOH instead of DNA template; Lane 7: PCR reaction using water instead of DNA template. Lane M contains DNA molecular size marker.