Fig. 5.

Comparison of tissue grinding duration on the amplification of DNA by PCR. Duplicate samples of three naturally infected leaf disks of Hosta ‘August Moon’ and Hosta ‘Midas Touch’ were ground in 0.5N NaOH for 10, 20 or 60 sec. Lanes 1 and 2: ‘August Moon’ 10 sec; Lanes 3 and 4: ‘August Moon’ 20 sec; Lanes 5 and 6: ‘August Moon’ 60 sec; Lanes 7 and 8: ‘Midas Touch’ 10 sec; Lanes 9 and 10: ‘Midas Touch’ 20 sec; Lanes 11 and 12: ‘Midas Touch’ 60 sec; Lane 13: known A. fragariae DNA; Lane 14: NaOH instead of DNA template. Lane M contains DNA molecular size marker.