FIGURE 2.

Analysis of TC and chromatin compaction: (a) Color-coded anisotropy profile of H2B-EGFP with solid lines marking TCs. (b) A typical line scan across a TC showing the TR-UTP intensity profile (solid, right axis) and the underlying H2B-EGFP anisotropy (r) profile (shaded, left axis). (c) Percentage sites which have underlying chromatin relatively more or less compacted then the neighborhood for TCs sites in HeLa cells under various hours of TSA treatment and for randomly chosen points inside the nucleus. (d) Cumulative distribution of fluorescence anisotropy of the nucleus (solid circles). The shaded bars represent anisotropy distribution below TC foci.