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Effective rotational correlation times (τR) from V2′ spectra (Fig. 6)
| Sample | L″/L | C′/C | τR (μs) |
|---|---|---|---|
| IASL-S1 | −0.26 ± 0.01 | ≤0.50 | |
| IASL-myosin | 0.47 ± 0.01 | 18 ± 1 | |
| MSL-S1 on actin + ATPγS(9) | 0.54 ± 0.05 | 24 ± 5 | |
| IASL-S1 on actin | 1.44 ± 0.02 | 560 ± 40 | |
| IASL-S1 in 20% PEG | 1.58 ± 0.02 | ≥1000 | |
| BSL-S1 | 0.18 ± 0.01 | ≤0.50 | |
| BSL-myosin | 0.51 ± 0.01 | 21 ± 1 | |
| BSL-S1 on actin | 1.46 ± 0.01 | 600 ± 20 | |
| BSL-S1 in 20% PEG | 1.60 ± 0.01 | ≥1000 |
Values are mean ± SE (n = 3-6).
