TABLE 1.
Summary of results from immunofluorescence microscopy studies
| Localization of nuclear envelope proteins relative to corresponding wild-type
|
||||
|---|---|---|---|---|
| WT localization | MAF LmnaL530P/L530P | MEF Lmna−/− | MEF Emd−/y | |
| Lamin A | Near inner nuclear membrane (54) | −* | − | + |
| Emerin | Inner and outer nuclear membrane (37,55) | − | − | − |
| Sun1 | Inner nuclear membrane (29) | − | + | + |
| Sun2 | Inner nuclear membrane (30) | − | + | + |
| Nesprin2g | Outer nuclear membrane (56) | − | ++ | + |
| Nesprin3 | Outer nuclear membrane (31) | − | − | + |
Symbols indicate the following in a majority of each laminopathic cell population: +, this protein has the same localization and density in/near the nuclear envelope as in wild-type littermates; ++, this protein has the same localization as in wild-type littermates, but with a higher density in the nuclear envelope; −, this protein has largely disappeared from the nuclear envelope; and *, see Fig. S1 in Data S1 for details about lamin A detection. See Fig. 1 for illustrative immunofluorescence micrographs.