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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2008 Jul;7(7):1900–1908. doi: 10.1158/1535-7163.MCT-08-0012

Figure 3. NDGA induces apoptosis of trastuzumab-refractory cells.

Figure 3

Figure 3

(A) SK-parental, SK-HRp2, and SK-HRc3 cells, and (B) BT-parental and BT-HRp3 cells were treated with 0, 25, 50, or 100 μM NDGA for 6 h. Immunoblotting (50 μg) was performed for PARP and caspase 3 cleavage. Actin served as a loading control. Blots were repeated at least twice to ensure reproducible results, and representative blots are shown. NDGA activated cleavage of PARP from the full-length 116-kDa form to the 89-kDa form, and of caspase 3 into the 19-kDa and 17-kDa forms, indicating induction of apoptosis by NDGA. The cleaved 89-kDa PARP and 19-kDa caspase 3 bands were quantitated using NIH ImageJ software.