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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2008 Jul 21;7(10):1624–1635. doi: 10.1016/j.dnarep.2008.06.006

Fig. 1.

Fig. 1

Recombination and NHEJ substrates pLB4 and pTNeo99-7. Each substrate contains a functional hygromycin resistance gene (hyg), used to select for stably transfected cells. The 18 bp recognition site for endonuclease I-SceI is underlined, and the sites of staggered cleavage by I-SceI are indicated. The orientation, with respect to transcription, of both the tk gene and/or tk-neo fusion gene in each substrate is from left to right as drawn. PCR primers AW85 and AW91 are indicated by short horizontal arrows and are located 1.4 kb apart in the tk-neo fusion gene. BamHI (B) and HindIII (H) sites are shown. See text for details.