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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2008 Jul 21;7(10):1624–1635. doi: 10.1016/j.dnarep.2008.06.006

Fig. 5.

Fig. 5

Representative Southern blot analysis of recombination events. Genomic DNA samples (8 µg) from G418R colonies recovered from pLB4/11 cells either treated with RTX (panel A) or untreated (panel B) were digested with BamHI and hybridized with a probe specific for HSV-1 tk to distinguish gene conversions from crossovers. Samples displaying both the 4.5 kb and 3.9 kb band were categorized as gene conversions while the samples displaying only the 3.9 kb band were categorized as crossovers (see Fig. 4). Cells treated with RTX displayed a significant shift toward gene conversion events.