Table 2.
Characterization of spontaneous recombination events
| Experiment number |
RTXa | Colonies Analyzed |
Gene Conversionsb | Crossoversb |
|---|---|---|---|---|
| 1 | − | 7 | 2 (2)c | 5 (4) |
| + | 12 | 12 (5) | 0 | |
| 2 | − | 10 | 2 (1) | 8 (4) |
| + | 9 | 9 (6) | 0 | |
| 3 | − | 4 | 1 (1) | 3 (2) |
| + | 12 | 12 (5) | 0 | |
a
In each expeiment, portions of cells from each subclone were untreated (−) or treated (+) for 24 hours with 1.5 nM RTX prior to plating into G418 selection as described in Materials and Methods.
b
Characterization of events as gene conversions (noncrossovers) versus crossovers was based on Southern blotting analysis of BamHI digests as described in the text.
c
Numbers in parentheses indicate the minimal number of independent recombination events.