Table 3.
Experiment No. |
RTXa | Cells platedb (105) |
Colonies recoveredc |
Colony frequencyd (10−3) |
---|---|---|---|---|
1 | − | 2.66 | 566 | 2.13 |
+ | 2.39 | 457 | 1.91 | |
2 | − | 1.70 | 589 | 3.46 |
+ | 1.05 | 438 | 4.17 |
Cells were untreated (−) or treated (+) with 1.5 nM RTX prior to electroporation with pSce, as described in Materials and Methods.
Total number of viable cells plated into G418 selection two days after electroporation with pSce.
Total number of G418R colonies recovered.
Number of G418R colonies divided by number of cells plated into G418.