Table 4.

Recovery of NHEJ events from cell line 9-2

Experiment	RTXa	Cells platedb (105)	Colonies recoveredc	Colony frequencyd (10−3)
1	−	17.62	3313	1.88
	+	12.89	3559	2.76
2	−	2.20	712	3.23
	+	1.60	544	3.40

^aCells were untreated (−) or treated (+) with 1.5 nM RTX prior to electroporation with pSce, as described in Materials and Methods.

^bTotal number of cells plated into G418 selection two days after electroporation with pSce, corrected for viability.

^cTotal number of G418^R colonies recovered.

^dNumber of G418^R colonies divided by number of viable cells plated into G418. In control experiments in which cell line 9-2 was mocked electroporated in phosphate buffered saline alone, colony frequency was less than 10⁻⁷.