Table 5.

NHEJ events recovered from cells treated with RTX

Clone name	Deletion size (bp)	Microhomologya
4ar	4	none
2ar	4b	none
5ar	4	none
6ar	7	a
8cr	7	none
7ar	10	a
6cr	22c	none
10ar	28	ggg
8ar	28	ggg
2br	31	c
5cr	49	none
3br	55	ctt
10br	100d	none
1ar	244	c
1br	244	gc
5br	403	c
9ar	1141	accc

^aFor NHEJ junctions displaying microhomology (segments of sequence identity) at the joined DNA termini, the actual sequence of microhomology shared between the joined termini is shown.

^bClone 2ar displayed a 4 bp deletion plus a run of 12 consecutive T residues inserted at the DSB site.

^cClone 6cr displayed a net deletion of 22 bp, including an insertion of a three bp sequence (tta) at the DSB site.

^dClone 10br displayed a net deletion of 100 bp, including an insertion of a 16 bp sequence (taataactgaaaacag) at the DSB site.