Table 6.
NHEJ events recovered from cells without RTX treatment
| Clone name | Deletion size (bp) | Microhomologya |
|---|---|---|
| 5c | 4 | none |
| 8b | 4 | none |
| 4b | 7 | a |
| 7a | 10 | gg |
| 8c | 22 | agct |
| 6b | 22 | agg |
| 5b | 33b | ctcab |
| 3a | 37 | g |
| 2b | 37 | none |
| 10b | 49 | ggc |
| 6a | 115 | gg |
| 9b | 169 | none |
| 3b | 186 | g |
| 1b | 199 | none |
| 3c | 214 | ac |
| 2a | 244 | gg |
| 4a | 244 | gggt |
| 9a | 256 | ggcc |
| 8a | 328 | none |
| 10a | 1087 | none |
a
For NHEJ junctions displaying microhomology (segments of sequence identity) at the joined DNA termini, the actual sequence of microhomology shared between the joined termini is shown.
b
Clone 5b displayed a 33 bp deletion plus an insertion of a 149 bp sequence originating from the hygromycin resistance gene (present in pTNeo99-7). The 4 bp microhomology (ctca) was located at the junction between the insert and the right-most DNA terminus from the DSB.