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Southern blot analysis reveals homozygous deletions of NF1 in primary OSCs. (A) Southern blot analysis of EcoRI-digested OSC DNA samples using three probes at different locations within the NF1 gene. To control for loading, the blots were rehybridized with a probe for DHX40, which lies telomeric to NF1 on chromosome 17q. (B) The relative location of each probe on chromosome 17 is shown in the diagram.
