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Expression of NF1 is reduced or absent in several ovarian carcinoma cell lines. (A) NF1 transcripts in the indicated cell lines were detected by Northern blot analysis, using expression of GAPDH as a loading control. Relative expression of NF1 in each cell line normalized to GAPDH (arbitrary units) is indicated. (B) Nf1 protein levels in the same cell lines were determined by Western blot analysis using an anti-Nf1 antibody. Detection of actin was used as a loading control.
