Figure 2.

Betel quid-induced cell migration was abolished in SFK-deficient cells. (A) Wild type MEFs and SYF, MEF cells lacking Src, Yes, and Fyn, were treated with BQ extract while being allowed to migrate for 3 hours and 4 hours, respectively. Betel quid extract could induce cell migration of MEF cells by about seven-fold, whereas no induction was observed in SYF cells. (B) Mouse embryonic fibroblast cells were treated with BQ extract for various periods as indicated, and phosphorylation of Src (pY416) was determined using Western blot analysis. Phosphorylation of SFKs at tyrosine 416 (Src 416) was increased in MEF cells 30 minutes after BQ extract treatment. Anti-SFKs antibody (Src2) detected total proteins of SFKs. GAPDH was the loading control.