Figure 3.

Slit2 enhances tumor apoptosis. (A) Athymic mice were injected subcutaneously with HT1080 tumor cells. Tumors were removed 2 weeks after inoculation, and paraffin-embedded sections were made. Apoptotic cells of tumor samples were determined by TUNEL assay. The number of apoptotic cells was counted microscopically in five fields. **P < .005. (B) HT1080 tumor tissues were collected, and tumor lysates were subjected to Western blot analysis. Bcl-xl, Cdk6, and Cyclin D1 were down-regulated in tumors of mice injected with Slit2-transfected HT1080 cells. (C) The relative levels of proteins in each sample were determined by Quantity one 1-D Analysis Software and were normalized to GAPDH.