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Slit2 inhibits tumor cell migration and invasion. (A) Migration of tumor cells was assessed by wound healing assay. Migration index was calculated as described in the Materials and Methods section. *P < .001. (B) Invasiveness of tumor cells was determined by using a two-chamber Matrigel system. Tumor invasion was evaluated at 24 hours after culture. The number of invaded cells was counted microscopically in 20 (HT1080) or 40 fields (A431). *P < .001.
