Figure 4.

Phorbol 12-myristate 13-acetate induces changes in the composition of chromatin-remodeling protein complexes at specific NFκB-p50 sites in the promoter of the KAI1 gene. (A) LNCaP cells were either treated with DMSO vehicle, 5 ng/ml IL-1β, and 20 nM PMA for 1 hour or treated with 10 µM Bim III before 20 nM PMA for the indicated times and then processed for ChIP analysis of proteins binding to the KAI1 promoter, as described in the Materials and Methods section. Data are representative of two independent experiments. (B) Samples from (A) were subsequently analyzed by two-step ChIP to determine interactions between specific proteins binding the KAI1 promoter in cells treated with PMA for 1 and 4 hours.