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. 2008 Dec;10(12):1470–1480. doi: 10.1593/neo.08442

Figure 1.

Figure 1

Endogenous PAX2 binds to WNT5A 5′-upstream region. (A) Western analysis of Pax2 protein expression in HEK293 cells nuclear extract. (B) Chromatin immunoprecipitation was performed using a polyclonal antibody directed against Pax2. The enriched chromatin was amplified using primers flanking the NACE enriched WNT5A 5′-upstream region. A specific band of an expected size (∼300 bp) was obtained in the sample treated with the anti-Pax2 antibody but not in samples treated with an anti-IgG or mock controls.