Figure 3.

Transactivation assays of the WNT5A 5′-upstream region. The ∼300-bp NACE-enriched fragment (AB) and subfragments A and B were cloned into the pGL-3 CMV luciferase vector. The three vectors were cotransfected with pcDNA-4/His/Max-PAX2 expression vector and pRL-CMV internal control plasmid into HEK293 cells. Dual-luciferase activity ratios were calculated relative to express-tagged PAX2 activation of P2BS, the PAX2 binding site reporter. Error bars, SEM.