Table 1.
Forward | Reverse |
5′-GAG GAG AAG CGC AGT CAA TC-3′ | 5′-GAA GAG GAA GAA CAC GCA CA-3′ |
5′-CCA CCT CGA GAT TTC ACG TA-3′ | 5′-TTT TCC TAC CTA TCT GCA TCA CC-3′ |
5′-GCC AGT GAT CCC TTG TCC T-3′ | 5′-GGG CAA GGT GAG AAG ACA GA-3′ |
5′-CAT CTG GCT CCT CCA TGA AT-3′ | 5′-GGT GGC ACC CAC TAC TTG-3′ |
5′-ATG AGT CCA ACA TGC AGA GA-3′ | 5′-GTA ACT GTA TCA GTG AAA AT-3′ |
5′-ATG AGT CCA ACA TGC AGA GA-3′ | 5′-GTT GGC AGG CAC AGC TGG TT-3′ |
5′-AAC CAG CTG TGC CTG CCA AC-3′ | 5′-GTA ACT GTA TCA GTG AAA AT-3′ |
The PCR conditions were optimized to amplify a specific band of expected size using DNA samples as templates. The NACE-enriched WNT5A 5′-upstream element (AB) was amplified totally or partially (subfragments A and B) before cloning.