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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Oct 9;377(1):236–241. doi: 10.1016/j.bbrc.2008.09.130

Fig. 4.

Fig. 4

SNM1A is a phosphorylation substrate of ATM in vitro and interacts with ATM in vivo. (A) The SQ and TQ sites present in the N-terminus (NT) region of SNM1A and in full-length SNM1B are shown. (B) An IP kinase assay shows that recombinant MBP-SNM1A-NT (SNM1A residues 1-393), but not SNM1B is an ATM substrate. Left side of each panel shows Coomassie blue stained gels, and right sides show autoradiograms. “Mock” indicates an immunoprecipitation performed with a nonspecific IgG. “NS” indicates a nonspecific substrate of ATM. (C) Immunoblot showing reciprocal co-IP of ATM and SNM1A with or without IR treatment (10 Gy).